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qPCR Amplification Curves

Fluorescence vs cycle for serial dilutions with Cq markers.

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{
  "data": [
    { "type":"scatter","mode":"lines","name":"10^6 copies","x":[15,17,19,21,23,25,27,29],"y":[0.05,0.1,0.4,1.5,4,8,11,12],"line":{"color":"#3666ff","width":2.5} },
    { "type":"scatter","mode":"lines","name":"10^5 copies","x":[18,20,22,24,26,28,30,32],"y":[0.05,0.1,0.4,1.5,4,8,11,12],"line":{"color":"#7c3aed","width":2.5} },
    { "type":"scatter","mode":"lines","name":"10^4 copies","x":[21,23,25,27,29,31,33,35],"y":[0.05,0.1,0.4,1.5,4,8,11,12],"line":{"color":"#ec4899","width":2.5} },
    { "type":"scatter","mode":"lines","name":"Threshold","x":[15,35],"y":[1,1],"line":{"color":"#dc2626","dash":"dash"} }
  ],
  "layout":{
    "title":"qPCR amplification — 10-fold dilution series",
    "xaxis":{"title":"Cycle number","range":[15,35]},
    "yaxis":{"title":"Relative fluorescence","type":"log"}
  }
}