Chromatography — retention, selectivity and efficiency

Diagnose a separation by examining retention, selectivity and peak width separately. Use a suitability mixture and replicate injections; changing one operating factor can trade resolution against analysis time.

ScienceChemistry & Physics Science syntax docs

Template previewScience

Rendering…

What this template contains

Source: 25 lines

Make it your own.

{
  "nodes": [
    { "id": "mix", "type": "rounded", "label": "Suitability mixture", "x": 40, "y": 200, "width": 150, "height": 104, "fill": "#ede9fe", "stroke": "#6d28d9" },
    { "id": "run", "type": "rounded", "label": "Separation conditions", "x": 215, "y": 200, "width": 150, "height": 104, "fill": "#fef3c7", "stroke": "#b45309" },
    { "id": "ret", "type": "card", "label": "Retention times", "x": 390, "y": 40, "width": 150, "height": 104, "fill": "#ccfbf1", "stroke": "#0f766e" },
    { "id": "width", "type": "card", "label": "Peak widths", "x": 390, "y": 360, "width": 150, "height": 104, "fill": "#ccfbf1", "stroke": "#0f766e" },
    { "id": "sel", "type": "rounded", "label": "Selectivity between peaks", "x": 565, "y": 40, "width": 150, "height": 104, "fill": "#fef3c7", "stroke": "#b45309" },
    { "id": "eff", "type": "rounded", "label": "Column efficiency", "x": 565, "y": 360, "width": 150, "height": 104, "fill": "#fef3c7", "stroke": "#b45309" },
    { "id": "res", "type": "card", "label": "Resolution and run time", "x": 740, "y": 200, "width": 150, "height": 104, "fill": "#ccfbf1", "stroke": "#0f766e" },
    { "id": "rep", "type": "octagon", "label": "Replicate injections", "x": 215, "y": 520, "width": 150, "height": 104, "fill": "#e0f2fe", "stroke": "#0369a1" },
    { "id": "opt", "type": "rounded", "label": "Adjust and recheck conditions", "x": 915, "y": 200, "width": 150, "height": 104, "fill": "#fce7f3", "stroke": "#be185d" }
  ],
  "edges": [
    { "id": "e0", "source": "mix", "target": "run", "label": "inject" },
    { "id": "e1", "source": "run", "target": "ret", "label": "record" },
    { "id": "e2", "source": "run", "target": "width", "label": "record" },
    { "id": "e3", "source": "ret", "target": "sel", "label": "compare retention" },
    { "id": "e4", "source": "width", "target": "eff", "label": "assess broadening" },
    { "id": "e5", "source": "sel", "target": "res", "label": "separation contribution" },
    { "id": "e6", "source": "eff", "target": "res", "label": "separation contribution" },
    { "id": "e7", "source": "rep", "target": "res", "label": "repeatability", "style": "dashed" },
    { "id": "e8", "source": "res", "target": "opt", "label": "trade-off" },
    { "id": "e9", "source": "opt", "target": "run", "label": "iterate", "style": "dashed" }
  ]
}