Chromatography — reversed-phase HPLC separation

{
  "nodes": [
    { "id": "sample", "type": "flask", "label": "Sample filtered to 0.22 µm", "x": 40, "y": 40, "width": 150, "height": 104, "fill": "#dbeafe", "stroke": "#1d4ed8", "bold": true },
    { "id": "resa", "type": "bottle", "label": "Reservoir A — water + 0.1% acid", "x": 215, "y": 40, "width": 150, "height": 104, "fill": "#ede9fe", "stroke": "#6d28d9" },
    { "id": "pump", "type": "motor", "label": "Binary pump — 1.0 mL/min gradient", "x": 390, "y": 40, "width": 150, "height": 104, "fill": "#e0f2fe", "stroke": "#0369a1", "bold": true },
    { "id": "inject", "type": "syringe", "label": "Autosampler — 10 µL loop", "x": 565, "y": 40, "width": 150, "height": 104, "fill": "#ede9fe", "stroke": "#6d28d9" },
    { "id": "column", "type": "cylinder-h", "label": "C18 column — 150 × 4.6 mm, 5 µm", "x": 740, "y": 40, "width": 150, "height": 104, "fill": "#fef3c7", "stroke": "#a16207", "bold": true },
    { "id": "detector", "type": "microscope", "label": "Diode-array detector at 254 nm", "x": 915, "y": 40, "width": 150, "height": 104, "fill": "#e0f2fe", "stroke": "#0369a1", "bold": true },
    { "id": "chrom", "type": "line-chart", "label": "Chromatogram — peaks against tR", "x": 1090, "y": 40, "width": 150, "height": 104, "fill": "#e0f2fe", "stroke": "#0369a1", "bold": true },
    { "id": "waste", "type": "bucket", "label": "Waste or fraction collector", "x": 1265, "y": 40, "width": 150, "height": 104, "fill": "#fee2e2", "stroke": "#b91c1c" },
    { "id": "prep", "type": "clipboard", "label": "Dissolve in the starting mobile phase", "x": 40, "y": 200, "width": 150, "height": 104, "fill": "#f1f5f9", "stroke": "#475569" },
    { "id": "resb", "type": "bottle", "label": "Reservoir B — acetonitrile", "x": 215, "y": 200, "width": 150, "height": 104, "fill": "#ede9fe", "stroke": "#6d28d9" },
    { "id": "degas", "type": "valve", "label": "Vacuum degasser", "x": 390, "y": 200, "width": 150, "height": 104, "fill": "#f1f5f9", "stroke": "#475569" },
    { "id": "guard", "type": "shield-check", "label": "Guard cartridge", "x": 740, "y": 200, "width": 150, "height": 104, "fill": "#f1f5f9", "stroke": "#475569" },
    { "id": "plates", "type": "clipboard", "label": "Plate count N = 5.54(tR/w½)²", "x": 915, "y": 200, "width": 150, "height": 104, "fill": "#f1f5f9", "stroke": "#475569" },
    { "id": "kfactor", "type": "bar-chart", "label": "Retention factor k = (tR − t0)/t0", "x": 1090, "y": 200, "width": 150, "height": 104, "fill": "#e0f2fe", "stroke": "#0369a1" },
    { "id": "mode", "type": "clipboard", "label": "Reversed phase — polar elutes first", "x": 565, "y": 360, "width": 150, "height": 104, "fill": "#f1f5f9", "stroke": "#475569" },
    { "id": "oven", "type": "gas-flame", "label": "Column oven held at 30 °C", "x": 740, "y": 360, "width": 150, "height": 104, "fill": "#ede9fe", "stroke": "#6d28d9" },
    { "id": "rs", "type": "target", "label": "Rs ≥ 1.5 — baseline separated", "x": 1090, "y": 360, "width": 150, "height": 104, "fill": "#dcfce7", "stroke": "#15803d", "bold": true }
  ],
  "edges": [
    { "id": "e1", "source": "prep", "target": "sample", "label": "prepare", "style": "dashed" },
    { "id": "e2", "source": "sample", "target": "inject", "label": "10 µL injected", "thickness": 2.5 },
    { "id": "e3", "source": "resa", "target": "degas" },
    { "id": "e4", "source": "resb", "target": "degas" },
    { "id": "e5", "source": "degas", "target": "pump" },
    { "id": "e6", "source": "pump", "target": "inject", "label": "mobile phase", "thickness": 2.5 },
    { "id": "e7", "source": "inject", "target": "guard" },
    { "id": "e8", "source": "guard", "target": "column", "thickness": 2.5 },
    { "id": "e9", "source": "oven", "target": "column", "label": "thermostatted", "style": "dashed" },
    { "id": "e10", "source": "mode", "target": "column", "label": "separation mode", "style": "dashed" },
    { "id": "e11", "source": "column", "target": "detector", "label": "eluent", "thickness": 2.5 },
    { "id": "e12", "source": "detector", "target": "chrom", "label": "absorbance trace", "thickness": 2.5 },
    { "id": "e13", "source": "detector", "target": "waste", "label": "to waste" },
    { "id": "e14", "source": "chrom", "target": "kfactor", "label": "per peak", "style": "dashed" },
    { "id": "e15", "source": "chrom", "target": "rs", "label": "per peak pair", "style": "dashed" },
    { "id": "e16", "source": "plates", "target": "column", "label": "efficiency", "style": "dashed" }
  ]
}