CRISPR-Cas9 Editing
From guide RNA design to edited cell line.
Rendering…
Make it your own.
flowchart LR
Target[Target DNA locus] --> Design[Design 20-nt guide RNA\nadjacent to PAM]
Design --> Synth[Synthesise gRNA +\nCas9 mRNA or RNP]
Synth --> Deliver["Deliver to cells<br/>(electroporation / LNP / viral)"]
Deliver --> Bind[Cas9 + gRNA binds DNA at PAM]
Bind --> Cut[Double-strand break]
Cut --> NHEJ{Repair pathway}
NHEJ -->|NHEJ| Ind[Indels — knock-out]
NHEJ -->|HDR + template| KI[Knock-in / point edit]
Ind --> Screen[Genotype + select clones]
KI --> Screen
Screen --> Pheno[Phenotype + downstream assays]