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Seed germination and salinity

Track germination and later growth as different outcomes under an illustrative salinity design. Typed FlowScript for laboratory biology. Keywords: experiment, assay, sample, protocol.

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Experiment flow — Illustrative Seed germination and salinityExperiment flow for Illustrative Seed germination and salinity. Samples: per_condition. 4 conditions: A Freshwater control; B Low salinity; C Moderate salinity; D Recovery transfer. Measured: Germination count; Root length; Survival count.EXPERIMENT FLOW4 GROUPSIllustrative Seed germination and salinityIndependent seed trays1 · SAMPLESPer Conditionn = 6 · 1 replicates2 · CONDITIONSAFreshwater control0 mM NaCl10 daysBLow salinity25 mM NaCl10 daysCModerate salinity75 mM NaCl10 daysDRecovery transfer75 mM then 0 mM NaCl10 days3 · MEASUREMENTASSAYGermination countPrespecified measurement; no observed resultssuppliedASSAYRoot lengthPrespecified measurement; no observed resultssuppliedASSAYSurvival countPrespecified measurement; no observed resultssupplied

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// Track germination and later growth as different outcomes under an illustrative salinity design.
// Synthetic worked example: all entities, schedules and measurements are illustrative.
// Every string below is EXAMPLE text from a fictional model: replace it.
// Replace assumptions and inputs before applying this model to actual work.

experiment design {
  title: "Illustrative Seed germination and salinity"
  model: "Independent seed trays"
}

// Six independent units per condition; technical repeats do not increase n.
sample per_condition {
  n: 6
  replicates: 1
}

condition condition_0 {
  title: "Freshwater control"
  dose: "0 mM NaCl"
  duration: "10 days"
}

condition condition_1 {
  title: "Low salinity"
  dose: "25 mM NaCl"
  duration: "10 days"
}

condition condition_2 {
  title: "Moderate salinity"
  dose: "75 mM NaCl"
  duration: "10 days"
}

condition condition_3 {
  title: "Recovery transfer"
  dose: "75 mM then 0 mM NaCl"
  duration: "10 days"
}

assay assay_0 {
  kind: "Germination count"
  readout: "Prespecified measurement; no observed results supplied"
}

assay assay_1 {
  kind: "Root length"
  readout: "Prespecified measurement; no observed results supplied"
}

assay assay_2 {
  kind: "Survival count"
  readout: "Prespecified measurement; no observed results supplied"
}

view design_flow: experiment_flow(design)