Enzyme kinetics — competing inhibition models
Compare initial rates across substrate and inhibitor concentrations. Fit competing models to untransformed rates and inspect residuals; a reciprocal plot alone should not determine the inhibition mechanism.
Rendering…
What this template contains
- Source
- 24 lines
Make it your own.
{
"nodes": [
{ "id": "sub", "type": "rounded", "label": "Substrate concentration series", "x": 40, "y": 40, "width": 150, "height": 104, "fill": "#ede9fe", "stroke": "#6d28d9" },
{ "id": "inh", "type": "rounded", "label": "Inhibitor concentration series", "x": 40, "y": 360, "width": 150, "height": 104, "fill": "#ede9fe", "stroke": "#6d28d9" },
{ "id": "rate", "type": "card", "label": "Initial-rate measurements", "x": 215, "y": 200, "width": 150, "height": 104, "fill": "#ccfbf1", "stroke": "#0f766e" },
{ "id": "blank", "type": "octagon", "label": "Blank and linear-time checks", "x": 215, "y": 520, "width": 150, "height": 104, "fill": "#e0f2fe", "stroke": "#0369a1" },
{ "id": "comp", "type": "rounded", "label": "Competitive model", "x": 390, "y": 40, "width": 150, "height": 104, "fill": "#fef3c7", "stroke": "#b45309" },
{ "id": "mixed", "type": "rounded", "label": "Mixed-inhibition model", "x": 390, "y": 360, "width": 150, "height": 104, "fill": "#fef3c7", "stroke": "#b45309" },
{ "id": "fit", "type": "rounded", "label": "Fit on original rate scale", "x": 565, "y": 200, "width": 150, "height": 104, "fill": "#fef3c7", "stroke": "#b45309" },
{ "id": "res", "type": "card", "label": "Residual / parameter diagnostics", "x": 740, "y": 200, "width": 150, "height": 104, "fill": "#ccfbf1", "stroke": "#0f766e" },
{ "id": "inf", "type": "rounded", "label": "Mechanism with uncertainty", "x": 915, "y": 200, "width": 150, "height": 104, "fill": "#fce7f3", "stroke": "#be185d" }
],
"edges": [
{ "id": "e0", "source": "sub", "target": "rate", "label": "vary" },
{ "id": "e1", "source": "inh", "target": "rate", "label": "vary" },
{ "id": "e2", "source": "blank", "target": "rate", "label": "validate", "style": "dashed" },
{ "id": "e3", "source": "rate", "target": "comp", "label": "candidate" },
{ "id": "e4", "source": "rate", "target": "mixed", "label": "candidate" },
{ "id": "e5", "source": "comp", "target": "fit", "label": "compare" },
{ "id": "e6", "source": "mixed", "target": "fit", "label": "compare" },
{ "id": "e7", "source": "fit", "target": "res", "label": "diagnose" },
{ "id": "e8", "source": "res", "target": "inf", "label": "bounded interpretation" }
]
}