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Mechanobiology — substrate to cellular response

Relate substrate mechanics and ligand presentation to cell adhesion, force generation and response. Changes in stiffness can be confounded by chemistry or geometry, so define matched controls.

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{
  "nodes": [
    { "id": "mat", "type": "rounded", "label": "Substrate stiffness and geometry", "x": 40, "y": 200, "width": 150, "height": 104, "fill": "#ede9fe", "stroke": "#6d28d9" },
    { "id": "lig", "type": "octagon", "label": "Matched ligand presentation", "x": 40, "y": 520, "width": 150, "height": 104, "fill": "#e0f2fe", "stroke": "#0369a1" },
    { "id": "adh", "type": "rounded", "label": "Cell adhesion complexes", "x": 215, "y": 200, "width": 150, "height": 104, "fill": "#fef3c7", "stroke": "#b45309" },
    { "id": "cyto", "type": "rounded", "label": "Cytoskeletal tension", "x": 390, "y": 200, "width": 150, "height": 104, "fill": "#fef3c7", "stroke": "#b45309" },
    { "id": "nuc", "type": "rounded", "label": "Nuclear / transcriptional response", "x": 565, "y": 200, "width": 150, "height": 104, "fill": "#fef3c7", "stroke": "#b45309" },
    { "id": "phen", "type": "card", "label": "Phenotypic readout", "x": 740, "y": 200, "width": 150, "height": 104, "fill": "#ccfbf1", "stroke": "#0f766e" },
    { "id": "force", "type": "card", "label": "Independent force measurement", "x": 390, "y": 520, "width": 150, "height": 104, "fill": "#ccfbf1", "stroke": "#0f766e" },
    { "id": "pert", "type": "octagon", "label": "Contractility perturbation", "x": 565, "y": 520, "width": 150, "height": 104, "fill": "#e0f2fe", "stroke": "#0369a1" },
    { "id": "infer", "type": "rounded", "label": "Mechanistic triangulation", "x": 915, "y": 200, "width": 150, "height": 104, "fill": "#fce7f3", "stroke": "#be185d" }
  ],
  "edges": [
    { "id": "e0", "source": "mat", "target": "adh", "label": "mechanical context" },
    { "id": "e1", "source": "lig", "target": "adh", "label": "chemical context", "style": "dashed" },
    { "id": "e2", "source": "adh", "target": "cyto", "label": "coupling" },
    { "id": "e3", "source": "cyto", "target": "nuc", "label": "transmission" },
    { "id": "e4", "source": "nuc", "target": "phen", "label": "response" },
    { "id": "e5", "source": "cyto", "target": "force", "label": "quantify" },
    { "id": "e6", "source": "pert", "target": "nuc", "label": "test dependence", "style": "dashed" },
    { "id": "e7", "source": "phen", "target": "infer", "label": "outcome" },
    { "id": "e8", "source": "force", "target": "infer", "label": "mechanics evidence" }
  ]
}