Enzyme temperature-response experiment

// Use initial-rate measurements and an inactive control to distinguish signal from background.
// Synthetic worked example: all entities, schedules and measurements are illustrative.
// Every string below is EXAMPLE text from a fictional model: replace it.
// Replace assumptions and inputs before applying this model to actual work.

experiment design {
  title: "Illustrative Enzyme temperature-response experiment"
  model: "Independent enzyme preparations"
}

// Six independent units per condition; technical repeats do not increase n.
sample per_condition {
  n: 6
  replicates: 1
}

condition condition_0 {
  title: "Low-temperature reference"
  dose: "10 degrees C"
  duration: "Initial-rate measurement window"
}

condition condition_1 {
  title: "Moderate temperature"
  dose: "25 degrees C"
  duration: "Initial-rate measurement window"
}

condition condition_2 {
  title: "High temperature"
  dose: "40 degrees C"
  duration: "Initial-rate measurement window"
}

condition condition_3 {
  title: "Heat-inactivated control"
  dose: "Inactive preparation"
  duration: "Initial-rate measurement window"
}

assay assay_0 {
  kind: "Initial reaction rate"
  readout: "Prespecified measurement; no observed results supplied"
}

assay assay_1 {
  kind: "Blank absorbance"
  readout: "Prespecified measurement; no observed results supplied"
}

assay assay_2 {
  kind: "Protein concentration"
  readout: "Prespecified measurement; no observed results supplied"
}

view design_flow: experiment_flow(design)