Biology — experimental design

// Biology vertical — experiments with samples, conditions, assays.
// The design view at the foot of the document draws through the
// experiment_flow renderer (experiment is its registered alias).

experiment drug_screen {
  title: "Compound screen — HDAC inhibitors on HCT116"
  model: "HCT116 colon-cancer cell line"
}

sample baseline {
  n: 6
  replicates: 3
}

condition vehicle {
  title: "DMSO vehicle control"
  dose: "0.1%"
  duration: "72 h"
}

condition compound_a {
  title: "Compound A"
  dose: "10 µM"
  duration: "72 h"
}

condition compound_b {
  title: "Compound B"
  dose: "10 µM"
  duration: "72 h"
}

assay viability {
  kind: "CellTiter-Glo"
  readout: "ATP luminescence, normalised to vehicle"
}

assay apoptosis {
  kind: "Annexin V / PI flow"
  readout: "% Annexin V+ cells"
}

view design: experiment_flow(drug_screen)