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Cell migration assay — motility versus proliferation

Interpret wound closure with time-resolved imaging, viability and proliferation controls. Separate collective migration from growth and use independent cultures as the unit of replication.

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{
  "nodes": [
    { "id": "seed", "type": "rounded", "label": "Independent cell cultures", "x": 40, "y": 200, "width": 150, "height": 104, "fill": "#ede9fe", "stroke": "#6d28d9" },
    { "id": "wound", "type": "rounded", "label": "Standardised initial gap", "x": 215, "y": 200, "width": 150, "height": 104, "fill": "#fef3c7", "stroke": "#b45309" },
    { "id": "img", "type": "card", "label": "Time-lapse imaging", "x": 390, "y": 200, "width": 150, "height": 104, "fill": "#ccfbf1", "stroke": "#0f766e" },
    { "id": "seg", "type": "rounded", "label": "Blinded segmentation", "x": 565, "y": 200, "width": 150, "height": 104, "fill": "#fef3c7", "stroke": "#b45309" },
    { "id": "area", "type": "card", "label": "Gap area over time", "x": 740, "y": 200, "width": 150, "height": 104, "fill": "#ccfbf1", "stroke": "#0f766e" },
    { "id": "pro", "type": "octagon", "label": "Proliferation control", "x": 390, "y": 520, "width": 150, "height": 104, "fill": "#e0f2fe", "stroke": "#0369a1" },
    { "id": "via", "type": "octagon", "label": "Viability control", "x": 565, "y": 520, "width": 150, "height": 104, "fill": "#e0f2fe", "stroke": "#0369a1" },
    { "id": "edge", "type": "card", "label": "Edge displacement tracking", "x": 740, "y": 520, "width": 150, "height": 104, "fill": "#ccfbf1", "stroke": "#0f766e" },
    { "id": "infer", "type": "rounded", "label": "Motility interpretation", "x": 915, "y": 200, "width": 150, "height": 104, "fill": "#fce7f3", "stroke": "#be185d" }
  ],
  "edges": [
    { "id": "e0", "source": "seed", "target": "wound", "label": "prepare" },
    { "id": "e1", "source": "wound", "target": "img", "label": "baseline and follow-up" },
    { "id": "e2", "source": "img", "target": "seg", "label": "images" },
    { "id": "e3", "source": "seg", "target": "area", "label": "closure curve" },
    { "id": "e4", "source": "seg", "target": "edge", "label": "trajectory" },
    { "id": "e5", "source": "area", "target": "infer", "label": "closure evidence" },
    { "id": "e6", "source": "edge", "target": "infer", "label": "movement evidence" },
    { "id": "e7", "source": "pro", "target": "infer", "label": "separate growth", "style": "dashed" },
    { "id": "e8", "source": "via", "target": "infer", "label": "exclude toxicity", "style": "dashed" }
  ]
}